Oleg Reva
University of Pretoria, South Africa
Title: Hidden activities of known antibiotics revealed by molecular docking of batumin, mupirocin and bacillaen.
Biography
Biography: Oleg Reva
Abstract
Drug induced reversion of antibiotic resistance is considered as a promising way to combat multidrug resistant infection that nowadays became a major threat to human health. Despite a general recognition of this opportunity, little studies were performed in the world to elucidate this phenomenon mainly because of the lack of reliable experimental models. An innovative anti-tuberculosis drug FS-1 was accepted for clinical use in Kazakhstan in 2015. Clinical trials and in vitro experiments revealed ability of this drug to induce reversion of antibiotic resistance in MDR-TB. A series of experiments on laboratory animals infected with the MDR-TB strain Mycobacterium tuberculosis SCAID 187.0 was performed for a systemic study of the drug resistance reversion phenomenon. The initial strain SCAID 187.0 was sequenced and the complete genome was deposited in NCBI (CP012506.1). Multiple Mtb isolates were collected at different stages of the experiment from the animals treated with traditional antibiotics with antibiotics supplemented with FS-1 in different concentrations and those which were left without treatment. The Mtb isolates were sequenced by Illumina and variant calling was performed. Bioinformatic analysis of the trends of genomic changes confirmed the theoretically predicted induced synergy mechanism of the drug resistance reversion. To our best knowledge, this experiment was the first systemic study of the drug resistance reversion phenomenon.